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Modelling production of extracellular polymeric substances in a pseudomonas aeruginosa chemostat culture
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2001
Year
Mucoid Pseudomonas AeruginosaEngineeringBiotransformationBioenergeticsBiotechnologyBiochemical EngineeringSynthetic BiologyMetabolic EngineeringMicrobial EcologyExtracellular Polymeric SubstancesEnvironmental MicrobiologyMicrobiologyChemostat CulturesMedicineMicrobiological Degradation
Formation of extracellular polymeric substances (EPSs) by mucoid Pseudomonas aeruginosa was investigated using literature data from chemostat cultures. The data were used to calibrate a product formation regime allowing substrate sufficient and endogenous EPS formation. Yield coefficients for both formation conditions were elucidated based on metabolic pathway analysis. Growth and non-growth related specific formation rates of 0.18 g CEPS/g Ccell/h and 0.03 1/h were estimated, respectively. The exogenous and endogenous EPS yield was found to be 0.77 g CEPS/g Cglu and 0.79 g CEPS/g Ccell, respectively. Being structurally equivalent to comprehensive maintenance models, this model allows for non-growth related product formation, showing the same quality of fit as previous models restricted to exogenous EPS precursors.