Production of 1,3-dihydroxyacetone from glycerol by Gluconobacter oxydans ZJB09112

Abstract

The culture variables were optimized to increase 1,3-dihydroxyacetone (DHA) production by Gluconobacter oxydans ZJB09112 in shake flask and bubble column bioreactor. After fermentation in the optimized medium (g/L: yeast extract 5, glycerol 2.5, mannitol 22.5, K(2)HPO(4) 0.5, KH(2)PO(4) 0.5, MgSO(4) x 7H(2)O 0.1, CaCO(3) 2.0, pH 5.0), when five times of glycerol feeding were made, 161.9 + or - 5.9 g/l of DHA was attained at 88.7 + or - 3.2% conversion rate of glycerol to DHA.