Humana Press eBooks · 2003 · 82 citations · 1 references
Life SciencesBiochemistryChemistryCellular MetabolismChemical KineticsMetabolismFatty Acid ChemistryFatty Acid OxidationLipid OxidationLipid PeroxidationOxidative Damage To BiomoleculesReactive Oxygen SpeciesFatty AcidsFree Radical KineticsFree Radical OxidationLipid HydroperoxidesLipid Oxidation KineticsLipid Peroxidation Of Polyunsaturated Fatty AcidsPolyunsaturated Fatty Acid OxidationThiobarbituric Acid Reactive Substances
Measurement of thiobarbituric acid reacting substances (TBARS), diene derivatives, and hydroperoxides are probably the most widely used procedures for evaluation of free radical-mediated oxidation of biological or model systems containing polyunsaturated fatty acids (PUFA). It is possible to write a sequence of rational reactions in which dienes formation comes first, followed by lipid hydroperoxides (LOOHs) production, and that terminates with fragmentation of the PUFA chains to carbonyl compounds that are TBARS. However, it appears that after the first few min, species of the three types co-exist even in simple suspensions of linoleic acid (,). These compounds are very reactive, especially in biological systems, and follow different rates of formation and conversion. Therefore, it is more informative to follow their kinetics of appearance and disappearance than to measure single time-points (, , ).
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Diane L. Tribble, J J van den Berg, Paul A. Motchnik et al. · Proceedings of the National Academy of Sciences · 1994 · 163 citations