Concepedia

Topo Cloning

TOPO Cloning is a molecular cloning method that exploits the topoisomerase I-mediated ligation of PCR products into linearized vectors, distinguishing itself from other cloning techniques by enabling directional or blunt-end cloning without the requirement for DNA ligase or restriction enzyme digestion.

16

Publications

422

Citations

76

Authors

36

Institutions

Publications per year

2017–2026

0

Parents

Publications

16

Cloning and sequencing of<i>Schizosaccharomyces pombe</i>DNA topoisomerase I gene, and effect of gene disruption

Tadashi Uemura, Kazuhiko Morino, Satoru Uzawa et al. · Nucleic Acids Research · 1987 · 85 citations · Full text

, , +12

TOPO TA is A‐OK: a test of phylogenetic bias in fungal environmental clone library construction

D. Lee Taylor, Ian Herriott, James M. Long et al. · Environmental Microbiology · 2007 · 73 citations

, , +16

Use of molecular cloning methods to map the distribution of epitopes on topoisomerase I (Scl‐70) recognized by sera of scleroderma patients

Peter D’Arpa, Helen White‐Cooper, Don W. Cleveland et al. · Arthritis & Rheumatism · 1990 · 47 citations

, , +17

Presence of an SAR‐like sequence in junction regions between an introduced transgene and genomic DNA of cultured tobacco cells: its effect on transformation frequency

Katsuya Shimizu, Misa Takahashi, Naoki Goshima et al. · The Plant Journal · 2001 · 29 citations · Full text

, , +13

Overview of Post Cohen‐Boyer Methods for Single Segment Cloning and for Multisegment DNA Assembly

Bryan Sands, Roger Brent · Current Protocols in Molecular Biology · 2016 · 25 citations · Full text

, , +17

1–5 of 16

Authors

76

  1. LH
    Lihua Hou

    Georgetown University, United States

    1 publications · 13 citations

    1

    h-index

  2. HK
    Habib Kaltoum

    Université Paris-Sud, France

    1 publications · 24 citations

    1

    h-index

  3. WW
    Wan‐Yi Wu

    National Taiwan University, Taiwan

    1 publications · 10 citations

    1

    h-index

  4. JS
    Junghan Song

    Seoul National University Bundang Hospital, South Korea

    1 publications · 14 citations

    1

    h-index

  5. GF
    Gui Feng

    Chinese Academy of Sciences, China

    1 publications · 13 citations

    1

    h-index

1–5 of 76

Institutions

36

  1. Kyoto University

    Kyoto, Japan

    1 h-index

    5 publications

    425 citations

  2. University of Alaska Fairbanks

    Fairbanks, United States

    1 h-index

    3 publications

    219 citations

  3. Institute of Mathematics and Computer Science

    Chisinau, Moldova

    1 h-index

    2 publications

    20 citations

  4. UConn Health

    Farmington, United States

    1 h-index

    1 publications

    47 citations

  5. Centre National de la Recherche Scientifique

    Paris, France

    1 h-index

    5 publications

    120 citations

  6. Florida International University

    Miami, United States

    1 h-index

    2 publications

    30 citations

  7. University of Regensburg

    Regensburg, Germany

    1 h-index

    1 publications

    24 citations

  8. Universidade de São Paulo

    São Paulo, Brazil

    1 h-index

    2 publications

    20 citations

1–8 of 36

Venues

  1. Nucleic Acids Research

    2 publications · 105 citations

    2

    h-index

  2. African Journal of Microbiology Research

    AJMR · 1 publications · 13 citations

    1

    h-index

  3. Plant Disease

    1 publications · 15 citations

    1

    h-index

  4. Arthritis & Rheumatism

    1 publications · 47 citations

    1

    h-index

  5. The Plant Journal

    1 publications · 29 citations

    1

    h-index

1–5