Concepedia

Specificity Optimization

Specificity Optimization is a methodological framework within the domain of engineered biorecognition elements that enhances the target selectivity of zinc finger nucleases by minimizing off-target binding through rational design or high-throughput screening of zinc finger arrays.

32

Publications

3.4K

Citations

152

Authors

62

Institutions

Publications per year

2017–2026

4

Parents

Publications

32

Rapid “Open-Source” Engineering of Customized Zinc-Finger Nucleases for Highly Efficient Gene Modification

Morgan L. Maeder, Stacey Thibodeau-Beganny, Anna Osiak et al. · Molecular Cell · 2008 · 709 citations · Full text

, , +16

Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection

Vikram Pattanayak, Cherie L. Ramirez, J. Keith Joung et al. · Nature Methods · 2011 · 454 citations · Full text

, , +11

A General Strategy for Selecting High-Affinity Zinc Finger Proteins for Diverse DNA Target Sites

Harvey A. Greisman, Carl O. Pabo · Science · 1997 · 330 citations

, , +17

Toward rules relating zinc finger protein sequences and DNA binding site preferences.

John R. Desjarlais, Jeremy M Berg · Proceedings of the National Academy of Sciences · 1992 · 222 citations · Full text

, , +14

Nuclear gene targeting in <i><scp>C</scp>hlamydomonas</i> using engineered zinc‐finger nucleases

André Greiner, Mayanka Awasthi, Suneel Kateriya et al. · The Plant Journal · 2012 · 185 citations · Full text

, , +15

1–5 of 32

Authors

152

  1. JK
    J. Keith Joung

    Massachusetts General Hospital, United States

    4 publications · 1.3K citations

    4

    h-index

  2. CO
    Carl O. Pabo

    Howard Hughes Medical Institute, United States

    3 publications · 661 citations

    3

    h-index

  3. VH
    Vera Helbl

    Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany

    2 publications · 88 citations

    2

    h-index

  4. TJ
    Tao Jiang

    Chinese Academy of Sciences, China

    2 publications · 736 citations

    2

    h-index

  5. DJ
    David J. Segal

    University of California, Davis, United States

    2 publications · 119 citations

    2

    h-index

1–5 of 152

Institutions

62

  1. Massachusetts General Hospital

    Boston, United States

    4 h-index

    21 publications

    8.2K citations

  2. Harvard University

    Cambridge, United States

    4 h-index

    22 publications

    6.4K citations

  3. Howard Hughes Medical Institute

    Chevy Chase, United States

    4 h-index

    13 publications

    3K citations

  4. Center for Cancer Research

    Bethesda, United States

    3 h-index

    15 publications

    6.7K citations

  5. Massachusetts Institute of Technology

    Cambridge, United States

    3 h-index

    11 publications

    2.1K citations

  6. Johns Hopkins University

    Baltimore, United States

    2 h-index

    4 publications

    504 citations

  7. Friedrich-Alexander-Universität Erlangen-Nürnberg

    Erlangen, Germany

    2 h-index

    5 publications

    225 citations

  8. Max Planck Institute for Molecular Genetics

    Berlin, Germany

    2 h-index

    2 publications

    77 citations

1–8 of 62

Venues

  1. Proceedings of the National Academy of Sciences

    PNAS · 4 publications · 561 citations

    4

    h-index

  2. Journal of Molecular Biology

    JMB · 4 publications · 288 citations

    4

    h-index

  3. Nucleic Acids Research

    3 publications · 299 citations

    3

    h-index

  4. Gene

    1 publications · 17 citations

    1

    h-index

  5. Science

    1 publications · 330 citations

    1

    h-index

1–5