Concepedia

Nuclease-mediated Knockout

Nuclease-Mediated Knockout is a gene knockout strategy that employs sequence-specific nucleases to induce targeted double-strand breaks in genomic DNA, thereby enabling the disruption of specific genes through error-prone repair mechanisms such as non-homologous end joining.

22

Publications

2.4K

Citations

169

Authors

50

Institutions

Publications per year

2017–2026

3

Parents

Publications

22

Gene-Targeted Mice Lacking the Trex1 (DNase III) 3′→5′ DNA Exonuclease Develop Inflammatory Myocarditis

Masashi Morita, Gordon Stamp, Peter Robins et al. · Molecular and Cellular Biology · 2004 · 382 citations · Full text

, , +17

Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases

Yolanda Santiago, Edmond M. Chan, Peiqi Liu et al. · Proceedings of the National Academy of Sciences · 2008 · 363 citations · Full text

, , +14

Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

James E. Dahlman, Omar O. Abudayyeh, Julia Joung et al. · Nature Biotechnology · 2015 · 288 citations · Full text

, , +9

Targeted Genome Modification in Mice Using Zinc-Finger Nucleases

Iara D Carbery, Diana Ji, Anne Harrington et al. · Genetics · 2010 · 278 citations · Full text

, , +16

In vivo cleavage of transgene donors promotes nuclease‐mediated targeted integration

Sandra Cristea, Yevgeniy Freyvert, Yolanda Santiago et al. · Biotechnology and Bioengineering · 2012 · 179 citations

, , +15

1–5 of 22

Authors

169

  1. FD
    Fyodor D. Urnov

    Sangamo BioSciences (United States), United States

    2 publications · 542 citations

    2

    h-index

  2. PD
    Philip D. Gregory

    Sangamo BioSciences (United States), United States

    2 publications · 542 citations

    2

    h-index

  3. YS
    Yolanda Santiago

    Sangamo BioSciences (United States), United States

    2 publications · 542 citations

    2

    h-index

  4. MC
    Michael C. Holmes

    University of Lancashire, United Kingdom

    2 publications · 542 citations

    2

    h-index

  5. WP
    Wenlei Peng

    Chinese University of Hong Kong, Hong Kong SAR China

    1 publications · 89 citations

    1

    h-index

1–5 of 169

Institutions

50

  1. Sangamo BioSciences (United States)

    Richmond, United States

    2 h-index

    19 publications

    5.6K citations

  2. Center for Systems Biology

    Boston, United States

    2 h-index

    2 publications

    385 citations

  3. Duke University

    Durham, United States

    2 h-index

    15 publications

    1.8K citations

  4. Harvard University

    Cambridge, United States

    2 h-index

    6 publications

    773 citations

  5. Merck & Co., Inc., Rahway, NJ, USA (United States)

    Rahway, United States

    1 h-index

    5 publications

    115 citations

  6. Chonnam National University

    Gwangju, South Korea

    1 h-index

    4 publications

    60 citations

  7. MRC Laboratory of Molecular Biology

    Cambridge, United Kingdom

    1 h-index

    1 publications

    363 citations

  8. Broad Institute

    Cambridge, United States

    1 h-index

    6 publications

    1.7K citations

1–8 of 50

Venues

  1. Proceedings of the National Academy of Sciences

    PNAS · 3 publications · 613 citations

    3

    h-index

  2. Genetics

    2 publications · 295 citations

    2

    h-index

  3. Molecular and Cellular Biology

    MCB · 2 publications · 455 citations

    2

    h-index

  4. PLoS ONE

    2 publications · 114 citations

    2

    h-index

  5. Molecular Therapy

    1 publications · 58 citations

    1

    h-index

1–5