Concepedia

Ligation-based Mutagenesis

Ligation-Based Mutagenesis is a site-directed mutagenesis method that relies on the enzymatic joining of synthetic oligonucleotides containing desired mutations into a linearized vector to generate precisely modified DNA sequences.

12

Publications

812

Citations

50

Authors

14

Institutions

Publications per year

2017–2026

0

Parents

Publications

12

PCR ligation mutagenesis in transformable streptococci: application and efficiency

Peter C. Lau, Chang K. Sung, Janet H. Lee et al. · Journal of Microbiological Methods · 2002 · 335 citations

, , +14

PCR-ligation-PCR mutagenesis: a protocol for creating gene fusions and mutations.

Stuart A. Ali, Alexander Steinkasserer · PubMed · 1995 · 135 citations

, , +14

A scalable pipeline for highly effective genetic modification of a malaria parasite

Claudia Pfander, Burcu Anar, Frank Schwach et al. · Nature Methods · 2011 · 107 citations

, , +14

Seamless Ligation Cloning Extract (SLiCE) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to SLiP Site-Directed Mutagenesis

Ken Motohashi · Methods in molecular biology · 2016 · 56 citations

, , +15

Molecular determinants of peculiar properties of a Pleurotus ostreatus laccase: Analysis by site-directed mutagenesis

Flavia Autore, Claudia Del Vecchio, Franca Fraternali et al. · Enzyme and Microbial Technology · 2009 · 48 citations

, , +9

1–5 of 12

Authors

50

  1. BA
    Bahram Arezi

    Agilent Technologies (United States), United States

    1 publications · 31 citations

    1

    h-index

  2. KM
    Ken Motohashi

    Tokyo Institute of Technology, Japan

    1 publications · 56 citations

    1

    h-index

  3. FA
    Flavia Autore

    King's College London, United Kingdom

    1 publications · 48 citations

    1

    h-index

  4. KV
    Katrin Volkmann

    Wellcome Sanger Institute, United Kingdom

    1 publications · 107 citations

    1

    h-index

  5. SA
    Stuart A. Ali

    University of the Witwatersrand, South Africa

    1 publications · 135 citations

    1

    h-index

1–5 of 50

Institutions

14

  1. University of Illinois Chicago

    Chicago, United States

    2 h-index

    3 publications

    682 citations

  2. Wellcome Sanger Institute

    Cambridge, United Kingdom

    1 h-index

    12 publications

    1.3K citations

  3. King Abdullah University of Science and Technology

    Jeddah, Saudi Arabia

    1 h-index

    1 publications

    107 citations

  4. King's College Hospital

    London, United Kingdom

    1 h-index

    2 publications

    96 citations

  5. University of Zurich

    Zurich, Switzerland

    1 h-index

    4 publications

    92 citations

  6. University of Toronto

    Toronto, Canada

    1 h-index

    2 publications

    670 citations

  7. Kyoto Sangyo University

    Kyoto, Japan

    1 h-index

    1 publications

    56 citations

  8. California Institute of Technology

    Pasadena, United States

    1 h-index

    1 publications

    12 citations

1–8 of 14

Venues

  1. Journal of Microbiological Methods

    2 publications · 363 citations

    2

    h-index

  2. PubMed

    Repository · 2 publications · 147 citations

    2

    h-index

  3. BioTechniques

    1 publications · 13 citations

    1

    h-index

  4. Nature Methods

    1 publications · 107 citations

    1

    h-index

  5. Methods in molecular biology

    Book series · 1 publications · 56 citations

    1

    h-index

1–5