Concepedia

Ligation-based Assembly

Ligation-Based Assembly is a molecular cloning technique that belongs to the class of recombinant DNA methods, specifically characterized by the covalent joining of compatible DNA fragments through phosphodiester bond formation catalyzed by DNA ligase, thereby enabling the construction of custom plasmid vectors.

27

Publications

1.3K

Citations

135

Authors

57

Institutions

Publications per year

2017–2026

8

Parents

Publications

27

Recombinant Spidroins Fully Replicate Primary Mechanical Properties of Natural Spider Silk

Christopher H. Bowen, Bin Dai, Cameron J. Sargent et al. · Biomacromolecules · 2018 · 235 citations · Full text

, , +11

Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly

Jennifer L. Ong, Rebecca Kucera, Bradley W. Langhorst et al. · ACS Synthetic Biology · 2018 · 214 citations · Full text

, , +16

BASIC: A New Biopart Assembly Standard for Idempotent Cloning Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology

Marko Storch, Arturo Casini, Ben Mackrow et al. · ACS Synthetic Biology · 2015 · 124 citations · Full text

, , +11

A microfluidic oligonucleotide synthesizer

Cheng-Chung Lee, Thomas M. Snyder, Stephen R. Quake · Nucleic Acids Research · 2010 · 97 citations · Full text

, , +17

Native Chemical Ligation–Photodesulfurization in Flow

Timothy S. Chisholm, Daniel Clayton, Luke J. Dowman et al. · Journal of the American Chemical Society · 2018 · 74 citations

, , +8

1–5 of 27

Authors

135

  1. GZ
    Guoping Zhao

    Chinese Academy of Sciences, China

    1 publications · 58 citations

    1

    h-index

  2. ZQ
    Zhongjun Qin

    Chinese Academy of Sciences, China

    1 publications · 58 citations

    1

    h-index

  3. JM
    Jane M. Michalski

    University of Maryland, Baltimore, United States

    1 publications · 58 citations

    1

    h-index

  4. PM
    Patti M. Aha

    Analog Devices (United States), United States

    1 publications · 32 citations

    1

    h-index

  5. CE
    Carl E. Hjelmen

    Texas A&M University, United States

    1 publications · 58 citations

    1

    h-index

1–5 of 135

Institutions

57

  1. Stanford University

    Stanford, United States

    2 h-index

    5 publications

    100 citations

  2. KTH Royal Institute of Technology

    Stockholm, Sweden

    2 h-index

    3 publications

    69 citations

  3. Peking University

    Beijing, China

    1 h-index

    8 publications

    336 citations

  4. Amyris (United States)

    Emeryville, United States

    1 h-index

    1 publications

    15 citations

  5. Jardin Botanique de Genève

    Geneva, Switzerland

    1 h-index

    1 publications

    18 citations

  6. Simon Fraser University

    Burnaby, Canada

    1 h-index

    1 publications

    29 citations

  7. Pharmaceutical Biotechnology (Czechia)

    Prague, Czechia

    1 h-index

    4 publications

    168 citations

  8. East China Normal University

    Shanghai, China

    1 h-index

    5 publications

    80 citations

1–8 of 57

Venues

  1. Nucleic Acids Research

    4 publications · 228 citations

    4

    h-index

  2. BioTechniques

    3 publications · 36 citations

    3

    h-index

  3. Methods in molecular biology

    Book series · 2 publications · 27 citations

    2

    h-index

  4. PLoS ONE

    2 publications · 68 citations

    2

    h-index

  5. ACS Synthetic Biology

    2 publications · 338 citations

    2

    h-index

1–5