Concepedia

Ligation Reaction Optimization

Ligation Reaction Optimization is a methodological framework in molecular biology that systematically enhances the efficiency and specificity of DNA ligation reactions by fine-tuning biochemical parameters, enzyme concentrations, and substrate ratios to achieve maximal recombinant yield under defined experimental conditions.

19

Publications

768

Citations

74

Authors

35

Institutions

Publications per year

2017–2026

4

Parents

Publications

19

A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB

Yanyan Lu, Feng‐Xia Liang, Xiaozhong Wang · Molecular Cell · 2014 · 247 citations · Full text

, , +10

Native Chemical Ligation at Asx-Cys, Glx-Cys: Chemical Synthesis and High-Resolution X-ray Structure of ShK Toxin by Racemic Protein Crystallography

Bobo Dang, Tomoya Kubota, Kalyaneswar Mandal et al. · Journal of the American Chemical Society · 2013 · 95 citations

, , +13

Oxo-ester Mediated Native Chemical Ligation: Concept and Applications

Qian Wan, Jin Chen, Yu Yuan et al. · Journal of the American Chemical Society · 2008 · 60 citations

, , +11

Optimization of sortase A ligation for flexible engineering of complex protein systems

Jess Li, Yue Zhang, Olivier Soubias et al. · Journal of Biological Chemistry · 2020 · 47 citations · Full text

, , +17

The fidelity of template-directed oligonucleotide ligation and its relevance to DNA computation

Kenneth D. James, Amy R. Boles, D. Henckel et al. · Nucleic Acids Research · 1998 · 32 citations · Full text

, , +12

1–5 of 19

Authors

74

  1. PS
    Pulaski Sp

    W.E. Upjohn Institute for Employment Research, United States

    1 publications · 14 citations

    1

    h-index

  2. DH
    D. Henckel

    The University of Texas at Austin, United States

    1 publications · 32 citations

    1

    h-index

  3. LL
    Lei Liu

    Tsinghua University, China

    1 publications · 31 citations

    1

    h-index

  4. KM
    Kalyaneswar Mandal

    University of Chicago, United States

    1 publications · 95 citations

    1

    h-index

  5. CA
    Casim A. Sarkar

    University of Pennsylvania, United States

    1 publications · 12 citations

    1

    h-index

1–5 of 74

Institutions

35

  1. Chinese Academy of Sciences

    Beijing, China

    2 h-index

    4 publications

    67 citations

  2. Pharmaceutical Biotechnology (Czechia)

    Prague, Czechia

    1 h-index

    1 publications

    13 citations

  3. University of California San Diego

    San Diego, United States

    1 h-index

    1 publications

    18 citations

  4. Deleted Institution

    1 h-index

    2 publications

    38 citations

  5. China Medical University

    Shenyang, China

    1 h-index

    5 publications

    105 citations

  6. Shenyang Agricultural University

    Shenyang, China

    1 h-index

    1 publications

    21 citations

  7. University of Southampton

    Southampton, United Kingdom

    1 h-index

    7 publications

    203 citations

  8. Columbia University

    New York, United States

    1 h-index

    4 publications

    240 citations

1–8 of 35

Venues

  1. Bioconjugate Chemistry

    2 publications · 59 citations

    3

    h-index

  2. Nucleic Acids Research

    2 publications · 50 citations

    2

    h-index

  3. Journal of the American Chemical Society

    JACS · 2 publications · 155 citations

    2

    h-index

  4. BioMed Research International

    1 publications · 19 citations

    1

    h-index

  5. ChemBioChem

    1 publications · 30 citations

    1

    h-index

1–5