Library Size Normalization is a preprocessing method in single-cell RNA-seq data analysis that scales gene expression counts by the total number of reads per cell to correct for technical variability in sequencing depth while preserving relative transcript abundance.
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Library Size Normalisation
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Accounting for technical noise in single-cell RNA-seq experiments
Philip Brennecke, Simon Anders, Jong Kim et al. · Nature Methods · 2013 · 1.1K citations · Full text
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