Concepedia

Knobs-into-holes Engineering

Knobs-Into-Holes Engineering is a protein engineering strategy in which complementary mutations are introduced into the Fc regions of two different antibody heavy chains to promote selective heterodimerization by creating steric compatibility between a protruding "knob" on one chain and a recessed "hole" on the other.

8

Publications

1.2K

Citations

95

Authors

25

Institutions

Publications per year

2017–2026

4

Variants

Knobs-into-holes Technology

Parents

Publications

8

‘Knobs-into-holes’ engineering of antibody C<sub>H</sub>3 domains for heavy chain heterodimerization

John Brady Ridgway, Leonard G. Presta, Paul Carter · Protein Engineering Design and Selection · 1996 · 802 citations

, , +15

Production of bispecific antibodies in “knobs-into-holes” using a cell-free expression system

Yiren Xu, Cuong Tran, Tyler H. Heibeck et al. · mAbs · 2014 · 97 citations · Full text

, , +17

Knobs-into-holes antibody production in mammalian cell lines reveals that asymmetric afucosylation is sufficient for full antibody-dependent cellular cytotoxicity

Whitney Shatz-Binder, Shan Chung, Bing Li et al. · mAbs · 2013 · 86 citations · Full text

, , +17

Efficient production of bispecific IgG of different isotypes and species of origin in single mammalian cells

Michael Dillon, Yiyuan Yin, Jianhui Zhou et al. · mAbs · 2016 · 76 citations · Full text

, , +17

Variable heavy–variable light domain and Fab-arm CrossMabs with charged residue exchanges to enforce correct light chain assembly

Sabine Imhof-Jung, Michael Mølhøj, J. Benz et al. · Protein Engineering Design and Selection · 2018 · 30 citations · Full text

, , +17

1–5 of 8

Authors

95

  1. WS
    Wendy Sandoval

    Gene Therapy Laboratory, France

    2 publications · 162 citations

    2

    h-index

  2. XY
    Xiaoping Yu

    Hunan Cancer Hospital, China

    1 publications · 22 citations

    1

    h-index

  3. WD
    Willie D. Wang

    Sutro Biopharma (United States), United States

    1 publications · 97 citations

    1

    h-index

  4. JL
    Jun Lü

    University of Tennessee Health Science Center, United States

    1 publications · 22 citations

    1

    h-index

  5. HZ
    Haifeng Zhao

    Shanxi Medical University, China

    1 publications · 22 citations

    1

    h-index

1–5 of 95

Institutions

25

  1. Guangzhou Regenerative Medicine and Health Guangdong Laboratory

    Guangzhou, China

    1 h-index

    1 publications

    22 citations

  2. Genentech

    San Francisco, United States

    1 h-index

    7 publications

    602 citations

  3. Shandong Institute of Business and Technology

    Yantai, China

    1 h-index

    1 publications

    22 citations

  4. Tokyo University of Pharmacy and Life Sciences

    Hachiōji, Japan

    1 h-index

    1 publications

    19 citations

  5. Guangzhou Eighth People's Hospital

    Guangzhou, China

    1 h-index

    1 publications

    22 citations

  6. The University of Tokyo

    Tokyo, Japan

    1 h-index

    6 publications

    114 citations

  7. Southern University of Science and Technology

    Shenzhen, China

    1 h-index

    5 publications

    110 citations

  8. KTH Royal Institute of Technology

    Stockholm, Sweden

    1 h-index

    1 publications

    19 citations

1–8 of 25

Venues

  1. mAbs

    3 publications · 259 citations

    3

    h-index

  2. Protein Engineering Design and Selection

    PEDS · 2 publications · 832 citations

    2

    h-index

  3. Journal of Biological Chemistry

    JBC · 1 publications · 21 citations

    1

    h-index

  4. Cell Discovery

    1 publications · 22 citations

    1

    h-index

  5. iScience

    1 publications · 19 citations

    1

    h-index

1–5