Assay Sensitivity Optimization is a methodological framework in diagnostic immunochemistry that systematically enhances the detection limit of monoclonal antibody-based assays by refining reagent concentrations, incubation parameters, and signal amplification techniques to maximize analytical sensitivity without compromising specificity.
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Anthony R. Mire‐Sluis, Yu Chen Barrett, Viswanath Devanarayan et al. · Journal of Immunological Methods · 2004 · 621 citations
Martha F. Kramer · Current Protocols in Molecular Biology · 2011 · 308 citations · Full text
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BIO · 14 publications · 270 citations
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