Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly→Pro substitution near the active site on its catalytic activity and stability

Ljudmila Kulakova, Andrey Galkin, Tôru Nakayama, +2

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Cold-active EsteraseBiochemistryNatural SciencesBiocatalysisEnzyme Catalysis +6

Cold-Active Serine Alkaline Protease from the Psychrotrophic Bacterium <i>Shewanella</i> Strain Ac10: Gene Cloning and Enzyme Purification and Characterization

Ljudmila Kulakova, Andrey Galkin, Tatsuo Kurihara, +2

Applied and Environmental Microbiology

Activation EnergySerine Alkaline ProteaseBiosynthesisBiochemistryProtein Folding +13

Pilot scale production and isolation of recombinant NAD+- and NADP+-specific formate dehydrogenases

Andrey Galkin, Vladimir V. Fedorchuk, P. Savitsky, +3

Biotechnology and Bioengineering

EngineeringMolecular BiologyNadp+-specific Formate DehydrogenasesBiosynthesisBioenergetics +16

Rational design of a trispecific antibody targeting the HIV-1 Env with elevated anti-viral activity

James J. Steinhardt, Javier Guenaga, Hannah L. Turner, +12

Nature Communications

Structural Insight into Arginine Degradation by Arginine Deiminase, an Antibacterial and Parasite Drug Target

Andrey Galkin, Liudmila Kulakova, Elif Sarıkaya, +3

Journal of Biological Chemistry

Crystal StructureL-arginine DeiminaseAntiparasitic AgentMolecular BiologyStructural Insight +20

Arginine Deiminase Uses an Active-Site Cysteine in Nucleophilic Catalysis of <scp>l</scp>-Arginine Hydrolysis

Xuefeng Lü, Andrey Galkin, Osnat Herzberg, +1

Journal of the American Chemical Society

Bioorganic ChemistryBiochemistryNatural SciencesBiocatalysisArginine Deiminase +9

Crystal Structures Representing the Michaelis Complex and the Thiouronium Reaction Intermediate of Pseudomonas aeruginosa Arginine Deiminase

Andrey Galkin, Xuefeng Lü, Debra Dunaway‐Mariano, +1

Journal of Biological Chemistry

L-arginine DeiminaseBacteriologyMolecular BiologyEnzymatic ModificationL-arginine Structure +14

Kinetic Analysis of <i>Pseudomonas aeruginosa</i> Arginine Deiminase Mutants and Alternate Substrates Provides Insight into Structural Determinants of Function

Xuefeng Lü, Ling Li, Rui Wu, +10

Biochemistry

L-arginine DeiminaseBacteriologyMolecular BiologyBacterial PathogensEnzymatic Modification +14

Structural Basis for Inactivation of Giardia lamblia Carbamate Kinase by Disulfiram

Andrey Galkin, Liudmila Kulakova, Kap Lim, +4

Journal of Biological Chemistry

Bacterial formate dehydrogenase. Increasing the enzyme thermal stability by hydrophobization of alpha‐helices

A. Rojkova, Andrey Galkin, Ludmila B. Kulakova, +3

FEBS Letters