Elicitation of tumor cell killing by CD8⁺ T cells is an effective therapeutic approach for cancer. In addition to using immune checkpoint blockade to reinvigorate existing but unresponsive tumor-specific T cells, alternative therapeutic approaches have been developed, including stimulation of polyclonal T cell cytolytic activity against tumors using bispecific T cell engager (BiTE) molecules that simultaneously engage the TCR complex and a tumor-associated Ag. BiTE molecules are efficacious against hematologic tumors and are currently being explored as an immunotherapy for solid tumors. To understand mechanisms regulating BiTE molecule--mediated CD8⁺ T cell activity against solid tumors, we sought to define human CD8⁺ T cell populations that efficiently respond to BiTE molecule stimulation and identify factors regulating their cytolytic activity. We find that human CD45RA⁺CCR7^- CD8⁺ T cells are highly responsive to BiTE molecule stimulation, are enriched in genes associated with cytolytic effector function, and express multiple unique inhibitory receptors, including leukocyte Ig-like receptor B1 (LILRB1). LILRB1 and programmed cell death protein 1 (PD1) were found to be expressed by distinct CD8⁺ T cell populations, suggesting different roles in regulating the antitumor response. Engaging LILRB1 with its ligand HLA-G on tumor cells significantly inhibited BiTE molecule-induced CD8⁺ T cell activation. Blockades of LILRB1 and PD1 induced greater CD8⁺ T cell activation than either treatment alone. Together, our data suggest that LILRB1 functions as a negative regulator of human CD8⁺ effector T cells and that blocking LILRB1 represents a unique strategy to enhance BiTE molecule therapeutic activity against solid tumors.

#	Concept	H-Index	Publications	Citations
1	Medicine	1	1	48
2	Immunology	1	1	48
3	Cancer Therapeutics	1	1	48
4	Immune Surveillance	1	1	48
5	Immunotherapy	1	1	48

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