21:I[432972,["/_next/static/chunks/bb923814e1f4d2e9.js","/_next/static/chunks/dd3ee9f5b7716ca6.js","/_next/static/chunks/4204a0ae82542ca8.js","/_next/static/chunks/1270dead247da766.js","/_next/static/chunks/6e1aa89a642f40be.js","/_next/static/chunks/2d7aa753e81324a8.js","/_next/static/chunks/8ffd0b71567920d8.js","/_next/static/chunks/67ea6dfd21f16c5f.js","/_next/static/chunks/24eb4379f68013fd.js","/_next/static/chunks/b8cd775ffdf935bd.js","/_next/static/chunks/983103cd9eb7b428.js","/_next/static/chunks/263f2cdcb9aa2341.js","/_next/static/chunks/294b9525248c8e5f.js"],"default"] 22:T45a,Cut flowers of a transgenic carnation (Dianthus caryophyllus L.) line (sACO-1 line), which was transformed with a carnation 1-aminocyclopropane-1-carboxylate (ACC) oxidase cDNA in sense orientation (sACO transgene), had a longer vase life than had flowers of the non-transformed plant and produced only a negligible amount of ethylene during natural senescence. The suppressed ethylene production in sACO-1 line was not accompanied with the accumulation of mRNA for ACC oxidase (DC-ACO1) gene in the gynoecium or mRNAs for DC-ACO1 and ACC synthase (DC-ACS1) genes in the petals. Previously, it was shown that in naturally senescing carnation flowers, ethylene is first produced from the gynoecium ; the evolved ethylene, acting as a diffusible signal which is perceived by petals, induces the expression of DC-ACO1 and DC-ACS1 genes that results in autocatalytic ethylene production in the petals (Shibuya et al., 2000). The results indicate that sACO transgene inhibits the expression of DC-ACO1, probably by cosuppression in the gynoecium, which then suppresses ethylene production in all flowers of sACO-1 line.23:T462,Carnation (Dianthus caryophyllus L. cv. Nora) plants, transformed with carnation ACC synthase (DC-ACS1) cDNA in sense or antisense orientation (the transgenes are sACS transgene and aACS transgene, respectively) by Agrobacterium-mediated gene transfer were produced. Cut flowers of all the transgenic lines obtained, 2 lines transformed with sACS transgene and 3 lines with aACS transgene, showed suppressed ethylene production during natural senescence as compared with flowers of the non-transformed control line. Among 5 transgenic lines, the sACS-1 line harboring sACS transgene had the severest reduction in ethylene production from flowers, and the flowers lost their vase-life with drying and discoloration in the rim of petals, the ethylene-independent deterioration of carnation flowers. DNA gel blot analysis revealed that the loci of sACS transgenes in the sACS-1 line were different from those in the 6-0-66 line, which is another transgenic line of different origin harboring sACS transgene, although both lines have two copies of the transgenes.24:T474,With 5 figures and 2 tables Abstract Use of resistance genes would be the most effective way to address Fusarium wilt caused by Fusarium oxysporum f.sp. melonis in melon. In this study, based on the BAC 31O16 sequence which contains the cluster of melon resistance gene homologue, we developed four new DNA markers linked to Fom‐1 , a dominant resistance gene against F. oxysporum f.sp. melonis races 0 and 2. Linkage analysis using 125 F 2 plants derived from the cross between P11 ( fom‐1fom‐1 ) and MR‐1 ( Fom‐1Fom‐1 ) indicated that Fom‐1 was located between CAPS markers C‐MRGH12 and 62‐CAPS at a distance of 0.4 and 1.2 cM, respectively. Map position of Fom‐1 was confirmed using another mapping population consisting of 104 recombinant inbred lines derived from the cross P11 × MR‐1. The analysis of 43 fixed lines and 27 commercial F 1 hybrids showed that the developed DNA markers linked to Fom‐1 correctly predicted the genotype in relation to the variety, suggesting that the region around the Fom‐1 locus is very polymorphic or there is no linkage disequilibrium between the locus and the marker loci.25:T431,DC-ACS1 is a gene for 1-aminocyclopropane-1-carboxylate (ACC) synthase which is expressed slightly in th

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