21:I[432972,["/_next/static/chunks/bb923814e1f4d2e9.js","/_next/static/chunks/dd3ee9f5b7716ca6.js","/_next/static/chunks/4204a0ae82542ca8.js","/_next/static/chunks/1270dead247da766.js","/_next/static/chunks/6e1aa89a642f40be.js","/_next/static/chunks/2d7aa753e81324a8.js","/_next/static/chunks/8ffd0b71567920d8.js","/_next/static/chunks/67ea6dfd21f16c5f.js","/_next/static/chunks/24eb4379f68013fd.js","/_next/static/chunks/b8cd775ffdf935bd.js","/_next/static/chunks/983103cd9eb7b428.js","/_next/static/chunks/263f2cdcb9aa2341.js","/_next/static/chunks/294b9525248c8e5f.js"],"default"] 22:T45a,Cut flowers of a transgenic carnation (Dianthus caryophyllus L.) line (sACO-1 line), which was transformed with a carnation 1-aminocyclopropane-1-carboxylate (ACC) oxidase cDNA in sense orientation (sACO transgene), had a longer vase life than had flowers of the non-transformed plant and produced only a negligible amount of ethylene during natural senescence. The suppressed ethylene production in sACO-1 line was not accompanied with the accumulation of mRNA for ACC oxidase (DC-ACO1) gene in the gynoecium or mRNAs for DC-ACO1 and ACC synthase (DC-ACS1) genes in the petals. Previously, it was shown that in naturally senescing carnation flowers, ethylene is first produced from the gynoecium ; the evolved ethylene, acting as a diffusible signal which is perceived by petals, induces the expression of DC-ACO1 and DC-ACS1 genes that results in autocatalytic ethylene production in the petals (Shibuya et al., 2000). The results indicate that sACO transgene inhibits the expression of DC-ACO1, probably by cosuppression in the gynoecium, which then suppresses ethylene production in all flowers of sACO-1 line.23:T462,Carnation (Dianthus caryophyllus L. cv. Nora) plants, transformed with carnation ACC synthase (DC-ACS1) cDNA in sense or antisense orientation (the transgenes are sACS transgene and aACS transgene, respectively) by Agrobacterium-mediated gene transfer were produced. Cut flowers of all the transgenic lines obtained, 2 lines transformed with sACS transgene and 3 lines with aACS transgene, showed suppressed ethylene production during natural senescence as compared with flowers of the non-transformed control line. Among 5 transgenic lines, the sACS-1 line harboring sACS transgene had the severest reduction in ethylene production from flowers, and the flowers lost their vase-life with drying and discoloration in the rim of petals, the ethylene-independent deterioration of carnation flowers. DNA gel blot analysis revealed that the loci of sACS transgenes in the sACS-1 line were different from those in the 6-0-66 line, which is another transgenic line of different origin harboring sACS transgene, although both lines have two copies of the transgenes.

#	Concept	H-Index	Publications	Citations
1	Biology	2	2	62
2	Engineering	1	1	27
3	Health Sciences	1	1	27
4	Medicine	1	1	35
5	Natural Sciences	1	1	35

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