Yulin Lestari

<p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;text-indent:14.15pt;line-height:normal;"><em><span style="font-size:9pt;">Xanthomonas oryzae</span></em><span style="font-size:9pt;"> pv. <em>oryzae</em> (<em>Xoo</em>), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten <em>Streptomyces</em> spp. isolates in suppressing <em>Xoo</em> disease was assessed <em>in planta</em> and <em>in vitro</em>. <em>In planta</em> experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both <em>Streptomyces</em> spp. and <em>Xoo</em>, and plants inoculated with only <em>Streptomyces</em> spp. Plants inoculated with <em>Xoo</em> and sprayed with a chemical bactericide, and plants inoculated with only <em>Xoo</em> served as positive controls, whereas plants not inoculated with either <em>Streptomyces</em> spp. or <em>Xoo</em> were used as negative controls. The results showed that the effect of endophytic <em>Streptomyces</em> spp. on BLB disease expressed as area under disease progress curve (AUDPC) was not significantly different to that on control plants (P > 0.05). However, plants inoculated with endophytic <em>Streptomyces</em> spp. were significantly taller and produced higher tiller number than control plants (P < 0.05). <em>Streptomyces </em>spp. isolate AB131-1 gave the highest plant height. <em>In vitro </em>studies on biocontrol mechanisms of selected <em>Streptomyces</em> spp. isolates showed that isolate LBR02 gave the highest inhibition activity on <em>Xoo</em> growth, followed by AB131-1 and AB131-2. Two isolates (AB131-1 and LBR02) were able to produce chitinase, phosphatase, and siderophore which included biocontrol characteristics. Morphological and colonization studies under SEM and light microscopy confirmed that the three isolates were endophytic <em>Streptomyces</em> spp. from different species. These studies found that the paddy plant which was inoculated with endophytic <em>Streptomyces</em> spp. AB131-1 and infected by <em>Xoo</em> could increase the height of plant and number of tillers.</span></p>

Ratih Dewi Hastuti, Yulin Lestari, Antonius Suwanto et al. · HAYATI Journal of Biosciences · 2013 · 82 citations · Full text

ALPHA-GLUCOSIDASE INHIBITOR ACTIVITY AND CHARACTERIZATION OF ENDOPHYTIC ACTINOMYCETES ISOLATED FROM SOME INDONESIAN DIABETIC MEDICINAL PLANTS

Sri Pujiyanto, Yulin Lestari, Antonius Suwanto et al. · 2012 · 51 citations

Alpha Glucosidase Inhibitor, Biochemistry, Bioassay-guided Isolation +13

Dietzia timorensis sp. nov., isolated from soil

Hideki Yamamura, Puspita Lisdiyanti, Roni Ridwan et al. · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 2009 · 31 citations

Botany, Actinomycete Strain, Dietzia Cinnamea +17

Metagenomic of Actinomycetes Based on 16S rRNA and nifH Genes in Soil and Roots of Four Indonesian Rice Cultivars Using PCR-DGGE

Mahyarudin Mahyarudin, İman Rusmana, Yulin Lestari · HAYATI Journal of Biosciences · 2015 · 28 citations · Full text

Biology, Microbial Diversity, Microbial Systematics +12

Actinokineospora baliensis sp. nov., Actinokineospora cibodasensis sp. nov. and Actinokineospora cianjurensis sp. nov., isolated from soil and plant litter

Puspita Lisdiyanti, Misa Otoguro, Shanti Ratnakomala et al. · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 2009 · 25 citations