Concepedia

Ratih Dewi Hastuti

RD

3

Publications

118

Citations

3

H-Index

2009

Active since

Affiliations

IPB University(Current)

Indonesian Institute of Sciences

National Institute of Technology and Evaluation

Ratih Dewi Hastuti is an author at IPB University specializing in natural sciences, environmental microbiology, and biology.

Top concepts

BiologyMedicineMicrobiologyNatural SciencesMicrobial EcologyPhylogenetic AnalysisEnvironmental Microbiology

Publications per year

2009–2013

3

Publications

3

Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants inoculated with only Streptomyces spp. Plants inoculated with Xoo and sprayed with a chemical bactericide, and plants inoculated with only Xoo served as positive controls, whereas plants not inoculated with either Streptomyces spp. or Xoo were used as negative controls. The results showed that the effect of endophytic Streptomyces spp. on BLB disease expressed as area under disease progress curve (AUDPC) was not significantly different to that on control plants (P &gt; 0.05). However, plants inoculated with endophytic Streptomyces spp. were significantly taller and produced higher tiller number than control plants (P &lt; 0.05). Streptomyces spp. isolate AB131-1 gave the highest plant height. In vitro studies on biocontrol mechanisms of selected Streptomyces spp. isolates showed that isolate LBR02 gave the highest inhibition activity on Xoo growth, followed by AB131-1 and AB131-2. Two isolates (AB131-1 and LBR02) were able to produce chitinase, phosphatase, and siderophore which included biocontrol characteristics. Morphological and colonization studies under SEM and light microscopy confirmed that the three isolates were endophytic Streptomyces spp. from different species. These studies found that the paddy plant which was inoculated with endophytic Streptomyces spp. AB131-1 and infected by Xoo could increase the height of plant and number of tillers.

Ratih Dewi Hastuti, Yulin Lestari, Antonius Suwanto et al. · HAYATI Journal of Biosciences · 2013 · 82 citations · Full text

Actinokineospora baliensis sp. nov., Actinokineospora cibodasensis sp. nov. and Actinokineospora cianjurensis sp. nov., isolated from soil and plant litter

Puspita Lisdiyanti, Misa Otoguro, Shanti Ratnakomala et al. · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 2009 · 25 citations

Streptomyces baliensis sp. nov., isolated from Balinese soil

Misa Otoguro, Shanti Ratnakomala, Yulin Lestari et al. · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 2009 · 11 citations

Biology, Microbial Diversity, Microbial Systematics +11

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