Disturbed blood flow (d-flow) has been known to induce changes of the cells in the arterial wall, increasing the risk of atherosclerosis. However, the heterogeneity of the vascular cell populations under d-flow remains less understood. To generate d-flow in vivo, partial carotid artery ligation (PCL) was performed. Seven days after ligation, single-cell RNA sequencing of nine left carotid arteries (LCA) from the PCL group (10,262 cells) or control group (14,580 cells) was applied and a single-cell atlas of gene expression was constructed. The integrated analysis identified 15 distinct carotid cell clusters, including 10 d-flow-relevant subpopulations. Among endothelial cells, at least four subpopulations were identified, including Klk8^hi ECs, Lrp1^hi ECs, Dkk2^hi ECs, and Cd36^hi ECs. Analysis of GSVA and single-cell trajectories indicated that the previously undescribed Dkk2^hi ECs subpopulation was mechanosensitive and potentially transformed from Klk8^hi ECs under d-flow. D-flow-induced Spp1^hi VSMCs subpopulation that appeared to be endowed with osteoblast differentiation, suggesting a role in arterial stiffness. Among the infiltrating cell subpopulations, Trem2^hi Mφ, Birc5^hi Mφ, DCs, CD4⁺ T cells, CXCR6⁺ T cells, NK cells, and granulocytes were identified under d-flow. Of note, the novel Birc5^hi Mφ was identified as a potential contributor to the accumulation of macrophages in atherosclerosis. Finally, Dkk2^hi ECs, and Cd36^hi ECs were also found in the proatherosclerotic area of the aorta where the d-flow occurs. In conclusion, we presented a comprehensive single-cell atlas of all cells in the carotid artery under d-flow, identified previously unrecognized cell subpopulations and their gene expression signatures, and suggested their specialized functions.

#	Concept	H-Index	Publications	Citations
1	Medicine	1	1	42
2	Natural Sciences	1	1	42
3	Cell Biology	1	1	42
4	Cell Signaling	1	1	42
5	Developmental Biology	1	1	42

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