Alejandro Vián

The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition

Benevides C. Pessela, César Mateo, Manuel Fuentes et al. · Enzyme and Microbial Technology · 2003 · 117 citations

Engineering, Biochemistry, Sepabeads Supports +11

<i>Escherichia</i> <i>coli</i> β-Galactosidase Recognizes a High-Energy Conformation of C-Lactose, a Nonhydrolizable Substrate Analogue. NMR and Modeling Studies of the Molecular Complex

Juan F. Espinosa, Emilio Montero, Alejandro Vián et al. · Journal of the American Chemical Society · 1998 · 95 citations

Nmr Spectroscopy, Biochemistry, Biomolecular Structure Prediction +14

Conformational Selection of Glycomimetics at Enzyme Catalytic Sites:  Experimental Demonstration of the Binding of Distinct High-Energy Distorted Conformations of <i>C</i>-, <i>S</i>-, and <i>O</i>-Glycosides by <i>E. </i><i>C</i><i>oli</i> β-Galactosidases

Alicia Garcı́a-Herrero, Esther Montero, José Muñoz-Muñoz et al. · Journal of the American Chemical Society · 2002 · 82 citations

Affinity chromatography of polyhistidine tagged enzymes

César Mateo, Gloria Fernández‐Lorente, Benevides C.C Pessela et al. · Journal of Chromatography A · 2001 · 81 citations

Affinity Chromatography, Biochemistry, Bioanalysis +5

Reversible immobilization of a thermophilic β-galactosidase via ionic adsorption on PEI-coated Sepabeads

Benevides C. Pessela, Roberto Fernández‐Lafuente, Manuel Fuentes et al. · Enzyme and Microbial Technology · 2003 · 80 citations

Chemical Engineering, Engineering, Biochemical Engineering +6