Daria Krefft

Thermostable proteins bioprocesses: The activity of restriction endonuclease-methyltransferase from Thermus thermophilus (RM.TthHB27I) cloned in Escherichia coli is critically affected by the codon composition of the synthetic gene

Daria Krefft, Aliaksei Papkov, Agnieszka Żylicz-Stachula et al. · PLoS ONE · 2017 · 44 citations · Full text

Life Sciences, Biotechnology, Microbiology +17

A vector-enzymatic DNA fragment amplification-expression technology for construction of artificial, concatemeric DNA, RNA and proteins for novel biomaterials, biomedical and industrial applications

Piotr M. Skowron, Natalia Krawczun, Joanna Żebrowska et al. · Materials Science and Engineering C · 2019 · 16 citations

Engineering, Biomedical Engineering, Biotechnology +10

T7-lac promoter vectors spontaneous derepression caused by plant-derived growth media may lead to serious expression problems: a systematic evaluation

Daria Krefft, Maciej Prusinowski, Paulina Maciszka et al. · Microbial Cell Factories · 2022 · 16 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17

Two-stage gene assembly/cloning of a member of the TspDTI subfamily of bifunctional restriction endonucleases, TthHB27I

Daria Krefft, Agnieszka Żylicz-Stachula, Ewa Mulkiewicz et al. · Journal of Biotechnology · 2014 · 11 citations

Life Sciences, Genetics, Molecular Biology +9

An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Piotr M. Skowron, Daria Krefft, Robert Brodzik et al. · Microbial Cell Factories · 2020 · 11 citations · Full text

Life Sciences, Biochemistry, Biotechnology +17