Efficiency of signalling through cytokine receptors depends critically on receptor orientation

Rashid Syed, Scott W. Reid, Cuiwei Li, +15

Nature

InflammationCytokineMolecular PhysiologySignal TransductionFunctional Selectivity +11

Characterization and Biological Effects of Recombinant Human Erythropoietin

Joan C. Egrie, T W Strickland, Julie A. Lane, +7

Immunobiology

Cell BiologyBlood CellBiological Effects

Dimerization of the Extracellular Domain of the Erythropoietin (EPO) Receptor by EPO:  One High-Affinity and One Low-Affinity Interaction

John S. Philo, Kenneth H. Aoki, Tsutomu Arakawa, +2

Biochemistry

Low-affinity InteractionExtracellular DomainSedimentation EquilibriumSedimentation Equilibrium DataCellular Physiology +17

The effect of carbohydrate on the structure and stability of erythropoietin.

Linda O. Narhi, Tsutomu Arakawa, Kenneth H. Aoki, +4

Journal of Biological Chemistry

Protein ChemistryM Guanidine HclProtein FunctionProtein AssemblyBiochemistry +12

NMR structure of human erythropoietin and a comparison with its receptor bound conformation

Janet C. Cheetham, D.M. Smith, Kenneth H. Aoki, +5

Nature Structural Biology

BiochemistryNmr StructureNatural SciencesMolecular BiologyHeme Signaling +3

S7.7 The role of carbohydrate on the biological activity of erythropoietin

Joan C. Egrie, James R. Grant, Dorothy Gillies, +2

Glycoconjugate Journal

Biological ActivityBiochemistryHeme HomeostasisNatural SciencesPhysiology +4

Biophysical Comparability of the Same Protein From Different Manufacturers: A Case Study Using Epoetin Alfa From Epogen® and Eprex®

Songpon Deechongkit, Kenneth H. Aoki, Sungae S. Park, +1

Journal of Pharmaceutical Sciences

Biophysical ComparabilityEngineeringBiochemistryNatural SciencesBioanalysis +3

Asn to Lys mutations at three sites which are N-glycosylated in the mammalian protein decrease the aggregation of Escherichia coli-derived erythropoietin

Linda O. Narhi, Tsutomu Arakawa, Kenneth H. Aoki, +4

Protein Engineering Design and Selection

Protein AssemblyGlycobiologyMolecular BiologyEscherichia ColiProtein Folding +17

Stabilization of Human Recombinant Erythropoietin through Interactions with the Highly Branched N-Glycans

Tadayuki Toyoda, Takanobu Itai, Tsutomu Arakawa, +2

The Journal of Biochemistry

ImmunologyGlycobiologyMolecular BiologyBlood CellPolysaccharide +16

Engineering a soluble extracellular erythropoietin receptor (EPObp) in Pichia pastoris to eliminate microheterogeneity, and its complex with erythropoietin

Hangjun Zhan, Beishan Liu, Scott W. Reid, +17

Protein Engineering Design and Selection

Signal TransductionEngineeringBiochemistryThiol ModificationG Protein-coupled Receptor +10