Kouta Sakaguchi

A targeted gene knockout method using a newly constructed temperature-sensitive plasmid mediated homologous recombination in Bifidobacterium longum

Kouta Sakaguchi, Jianlong He, Saori Tani et al. · Applied Microbiology and Biotechnology · 2012 · 62 citations

Temperature-sensitive Plasmid, Engineering, Homologous Recombination +9

Acquired Tolerance to Oxidative Stress in Bifidobacterium longum 105-A via Expression of a Catalase Gene

Jianlong He, Kouta Sakaguchi, Tohru Suzuki · Applied and Environmental Microbiology · 2012 · 32 citations · Full text

Protection Level, Bacteriology, Molecular Biology +16

The <i>pyrE</i> Gene as a Bidirectional Selection Marker in <i>Bifidobacterium Longum</i> 105-A

Kouta Sakaguchi, Nobutaka FUNAOKA, Saori Tani et al. · Bioscience of Microbiota Food and Health · 2013 · 28 citations · Full text

Engineering, Genetics, Bacteriology +16

Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A

Jianlong He, Kouta Sakaguchi, Tohru Suzuki · Journal of Bioscience and Bioengineering · 2012 · 23 citations

Spacer Length, Initiation Codon, Biochemistry +10

Levansucrase from Leuconostoc mesenteroides NTM048 produces a levan exopolysaccharide with immunomodulating activity

Ryuichi Ishida, Kouta Sakaguchi, Chiaki Matsuzaki et al. · Biotechnology Letters · 2016 · 21 citations

Immunomodulating Activity, Levan Exopolysaccharide, Immunology +4