[11] Transposon Tn5 mutagenesis to map genes

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +11

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

Reinhard Simon, U. B. Priefer, Alfred Pühler · Bio/Technology · 1983 · 7.3K citations

Life Sciences, Genetics, Molecular Genetics +12

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

Francisco Bolívar, Rodriguez Rl, Greene Pj et al. · PubMed · 1992 · 4.7K citations

Life Sciences, Biotechnology, Genetic Engineering +8

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

Annie Chang, Stanley N. Cohen · Journal of Bacteriology · 1978 · 4.3K citations · Full text

Life Sciences, Genetics, Molecular Biology +17