Characterization of bovine rod outer segment G-protein.

Abstract

A simple modified procedure is described for isolating and purifying peripherally bound membrane proteins from bovine rod outer segment disks.The methods yield milligram quantities of G-protein and cGMP phosphodiesterase which are suitable for reconstitution with membranes containing visual pigments.The properties of cGMP phosphodiesterase have been previously characterized (Baehr, W.,