Journal of Biological Chemistry · 1982 · 281 citations · 43 references
Life SciencesBiochemistryMolecular BiologyMembrane BiochemistryMembrane BiophysicsProtein BiochemistryMembrane ProteinsProtein PurificationProtein SeparationMembrane Protein ExtractionMembrane Protein PhosphorylationMembrane Protein PurificationMembrane Protein SeparationOuter Membrane ProteinsPeripheral Membrane Proteins
A simple modified procedure is described for isolating and purifying peripherally bound membrane proteins from bovine rod outer segment disks.The methods yield milligram quantities of G-protein and cGMP phosphodiesterase which are suitable for reconstitution with membranes containing visual pigments.The properties of cGMP phosphodiesterase have been previously characterized (Baehr, W.,
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Ulrich K. Laemmli · Nature · 1970 · 251.6K citations