Use of Micro Technique for Serum Neutralization and Virus Identification

Abstract

THE MICEO-TITEATION technique for serologic tests was first described in the Hungarian literature by Takatsy and associates (1).Sever (2) modified the technique and applied it to complement fixation, hemagglu¬ tination, hemagglutination-inhibition, and met- abolic-inhibition tests.Eosenbaum and co- workers (3) further applied the technique to the serum neutralization test.Their results in¬ dicated that serum neutralization by micro tech¬ nique reduced time, material, and effort and agreed well with results obtained by conven¬ tional tissue-culture tube methods.Our appli¬ cation of this method to the determination of neutralizing antibody titers, in paired serums, in a group of children who had received attenuated type I poliovirus vaccine produced results sim¬ ilar to those of Eosenbaum and associates (3).We also applied this micro method to the iden¬ tification of polioviruses isolated from another group of children who had received trivalent attenuated poliovirus vaccine.