SPECIFIC STAINING OF VARIOUS BACTERIA WITH A SINGLE FLUORESCENT ANTIGLOBULIN

Abstract

sensitive, and specific fluorescent antibody tech-nique for staining viruses in tissue sections. They used a direct staining procedure in which a spe-cific fluorescent antibody preparation is applied directly to the cell being studied. In the indirect procedure (Weller and Coons, 1954), the virus is first reacted with a specific nonfluorescent anti-serum prepared in the rabbit and then reacted with fluorescent antiglobulin prepared against rabbit globulin. The direct staining procedure was adapted to the staining of bacteria by Moody et al. (1956) who used fluorescein-labeled anti-serum to identify individual cells of Malleomyces pseudomallei on glass slides. Fluorescent antibody preparations have been used to identify Hemo-philus pertussis (de Repentigny and Frappier, 1956), Malleomyces pseudomallei (Thomason et al., 1956), and Salmonella species (Thomason et al.,