Isoelectric focusing, blotting and probing methods for detection and identification of monoclonal proteins.

Abstract

Isoelectric focusing (IEF) is a technique of exquisite resolution and high sensitivity. When applied to human biological fluids using conventional protein stains it is capable of detecting down to about 100 mg of protein/L. When combined with blotting and probing techniques it can get down to less than 1 mg/L. The exquisite resolution enables a greater discrimination between the various immunoglobulin abnormalities encountered in the clinical laboratory.