Regulation of MAP Kinase Signaling by Calcium

Abstract

Mitogen-activated protein kinase (MAPK) signaling influences a variety of cellular responses, ranging from stimulation of cell proliferation to induction of senescence and/or apoptosis. Ca(2+) is a ubiquitous intracellular signaling molecule that controls multiple processes in cells. Published evidence has identified both direct and indirect interactions between the Ca(2+) and MAPK signaling pathways. Here, we describe assays to accurately determine the effect of changes in intracellular Ca(2+) concentration on MAPK activation.