Polyadenylic acid-polyuridylic acid (poly A : U) and experimental murine brucellosis. I. Effect of single and double-stranded polynucleotides on Brucella abortus in vivo and in vitro.

Abstract

2'-Deoxy-3',5'-di-O-acetyl adenosine crystallizes in the orthorhombic space group P212121, and the cell dimensions are a = 7.768(1) Å, b = 12.890(1) Å, c = 15.495(1) Å; Z = 4 molecules per cell. Least-squares refinement converged at R = 0.054 for 1752 observed reflections. The adenine bases are linked via N6—H … N1 and N6—H … N7 hydrogen bonds, in such a way that infinite one-dimensional chains are formed. Comments are made on the fact that this structure is entirely different from the parallel dimer that was found for 3',5'-di-O-acetyl thymidine. Furthermore, the conformational preferences of the title compound in aqueous solution have been determined with 300- and 500-MHz 1H nmr. It is found that the X-ray structure and the solution conformation are essentially similar, except for the C4'—C5' conformation, that is ¿- in the solid state, and predominantly ¿+ in solution.