Influence of variety and extraction solvent on antibacterial activity of roselle (Hibiscus sabdariffa L.) calyxes

Abstract

An analysis was done on the antimicrobial activity of calyx extracts from five Hibiscussabdariffa varieties against Salmonella typhimurium and Salmonella choleraesuis. Calyx extracts produced with water, ethanol and methanol were applied to Salmonella cultures. All extracts exhibited antimicrobial activity against both Salmonella serotypes. The antimicrobial effect was highest in the variety named as “Alma blanca” (in which anthocyanin production has been almost completely suppressed). Extract pH had a significant (p<0.05) impact on extract antimicrobial effect. The variety had influence in the antimicrobial activity of H.sabdariffa calices.   Key words: Antimicrobial, Choleraesuis, plant extracts, Salmonella typhimurium, Hibiscussabdariffa.