Nitrogen-14 nuclear magnetic resonance spectroscopy as a probe of lipid bilayer headgroup structure.

Abstract

Nitrogen-14 NMR spectra were obtained at 26.0 M H z (corresponding to a magnetic field strength of 8.5 Tesla), using a "home-built" spectrometer and data system based on a LSI-I1 minicomputer.A two-pulse echo technique (11) using 90" pulse widths of -9 ps was used to record the 14N spectra.We used the "home-built" medium field spectrometer described previously (12) to record deuterium spectra.Single phase detection and a spectrum reverse technique ((Nicolet software package FT-74) was used to obtain an effective 1OC kHz spectral width.The deuterium 90" pulse width was 6 to 7 p, and ' The abbreviations used are: NMR, nuclear magnetic resonance.