An acoustofluidic embedding platform for rapid multiphase microparticle injection

Abstract

Droplet manipulation technologies play a critical role in many aspects of biochemical research, including in complex reaction assays useful for drug delivery, for building artificial cells, and in synthetic biology. While advancements have been made in manipulating liquid droplets, the capability to freely and dynamically manipulate solid objects across aqueous and oil phases remains unexplored. Here, we develop an acoustofluidic frequency-associated microsphere embedding platform, which enables microscale rapid injection of microparticles from a fluorinated oil into aqueous droplets. By observing different embedding mechanisms at low and high acoustic frequencies, we establish a theoretical model and practical principles for cross-phase manipulations. The proposed system not only enables multi-phase manipulation but also provides contactless control of specific microparticles within various distinctive phases. We demonstrate the acoustic-driven embedding and subsequent on-demand disassembly of hydrogel microspheres. This system indicates potential for reagent delivery and molecule capture applications. It enhances existing droplet manipulation technologies by enabling both multi-phase and cross-phase operations, paving the way for solid-liquid interaction studies in artificial cell research. The capability for intricate multi-phase loading, transport, and reactions offers promising implications for various fields, including in-droplet biochemical assays, drug delivery, and synthetic biology. Cross-phase manipulation holds potential for applications in synthetic biology and drug delivery. Here, authors present an acoustofluidic platform that enables rapid embedding of microparticles from an oil phase into aqueous droplets, offering an effective tool for studying cellular multiphase interactions and related phenomena.