Racial inequities and rare CFTR variants: Impact on cystic fibrosis diagnosis and treatment

Abstract

Cystic fibrosis (CF) has been traditionally viewed as a disease that primarily affects White individuals. The disorder results from mutations in the CF transmembrane conductance regulator (CFTR), with varying incidence of CF reported among Black, Indigenous, and People of Color (BIPOC). However, CF occurs among all races, ethnicities, and geographic ancestries, and BIPOC patients typically exhibit worse clinical outcomes. These populations are more likely to carry rare CFTR variants omitted from newborn screening panels, leading to disparities in care such as delayed diagnosis and treatment. In this study, we present a case-in-point describing an individual of Gambian descent identified with CF. Patient genotype includes a premature termination codon (PTC) (c.2353C > T) and previously undescribed single nucleotide deletion (c.1970delG), arguing against effectiveness of currently available CFTR modulator-based interventions. Strategies for overcoming these two variants will likely include combinations of PTC suppressors, nonsense mediated decay inhibitors, and/or alternative approaches (e.g. gene editing). Investigations such as the present study establish a foundation from which therapeutic treatments may be developed. Importantly, c.2353C > T and c.1970delG were not detected in the patient by traditional CFTR screening panels, which include an implicit racial and ethnic diagnostic bias as these tests are comprised of mutations largely observed in people of European ancestry. We suggest that next-generation sequencing of CFTR should be utilized to confirm or exclude a CF diagnosis, in order to equitably serve BIPOC individuals. Additional epidemiologic data, basic science investigations, and translational work are imperative for improving understanding of disease prevalence and progression, CFTR variant frequency, genotype-phenotype correlation, pharmacologic responsiveness, and personalized medicine approaches for patients with African ancestry and other historically understudied geographic lineages