Facile synthesis and optical characterization of selenium nanoparticles synthesized using <i>Clitoria ternatea</i> and <i>Zingiber officinale</i> : <i>in vitro</i> biomedical evaluation of antioxidant potential and antibacterial activity against caries-causing microbes

Abstract

In this study, selenium nanoparticles will be produced with Clitoria ternatea and Zingiber officinale, and their efficacy as a natural anti-cariogenic agent will be assessed. To do this, a variety of analysis and evaluation techniques have been used, such as UV-Vis spectroscopy to characterize the synthesized selenium nanoparticles that has absorption band at 440 nm. XRD of the Se nanoparticle were analysed and were found to be crystalline in nature with particles size of 36 nm. SEM images confirmed that Se nanoparticles were flaky structured. The antibacterial activity test to assess the selenium nanoparticles activity against dental caries causing bacteria and the zone of inhibition of the nanoparticles were found. Cytoplasmic leakage assay was done to govern the range of damage instigated by the synthesized selenium nanoparticles to bacterial cell membranes and time kill curve assay represents the time taken by the synthesized selenium nanoparticles to kill the cariogenic bacteria.