Copper-induced diurnal hepatic toxicity is associated with <i>Cry2</i> and <i>Per1</i> in mice

Abstract

BACKGROUND: This study aimed to investigate diurnal variations in copper-induced hepatic toxicity and the molecular mechanisms underlying this chronotoxicity. METHODS: treatment. We examined copper homeostasis- and apoptosis-related genes under clock genes overexpression. RESULTS: -induced inhibition of Hepa1-6 cell viability. Moreover, we found that the overexpression of Cry2 and Per1 regulates cleaved caspase-3 by modulating the copper transporter genes ATP7B and CTR1. CONCLUSION: -induced diurnal toxicity is associated with Cry2 and Per1 expression through the regulation of copper transporter genes in mice.