High Bioaffinity Controllable Assembly Nanocarrier UiO-66-NH₂@Quantum Dot-Based Immunochromatographic Assay for Simultaneous Detection of Five Mycotoxins in Cereals and Feed

Abstract

Herein, the UiO-66-NH₂@quantum dot (NU66@QD) was synthesized with excellent fluorescence intensity and biocompatibility, which was used to develop a multiple immunochromatographic assay (ICA) for the detection of aflatoxin B₁ (AFB₁), fumonisin B₁ (FB₁), deoxynivalenol (DON), T-2 toxins (T-2), and zearalenone (ZEN) in cereals and feed. Five monoclonal antibodies and NU66@QD were efficiently labeled by a one-step mixed method to form a multiple detection probe. The limits of detection of the proposed NU66@QD-ICA for AFB₁/FB₁/DON/T-2/ZEN were 0.04/0.28/0.25/0.09/0.08 μg/kg. The recoveries ranged from 82.83-117.44%, with the coefficient of variation from 2.88-11.80%. A parallel analysis in 35 naturally contaminated cereal and feed samples was confirmed by LC-MS/MS, and the results showed a good correlation (<i>R</i>² > 0.9), indicating the practical reliability of the multiple NU66@QD-ICA. Overall, the introduction of the novel nanomaterial NU66@QD provides a highly sensitive and efficient multiplex detection strategy for the development of ICA.