Analytical Chemistry · 2023 · 31 citations · 41 references
Bacterial infections seriously harm human health and cause many severe diseases, which triggered urgent demands to exploit specific and sensitive biosensor strategies for <i>Staphylococcus aureus</i> detection. Here, a colorimetric and photoelectrochemical dual-mode biosensor for <i>S. aureus</i> assay based on FePor-TPA was constructed. 2D FePor-TPA thin film and its bulk powder (FePor-TPA) were synthesized by in situ growth on ITO and a solvothermal condition, respectively, both of which exhibited excellent peroxidase-like and catalase-like activity, originating from their metalloporphyrin linkers. Benefiting from the <i>in situ</i> growth on ITO electrodes, the 2D FePor-TPA thin film also possessed a more ordered stacking mode and in turn exhibited good electrical conductivity, stable initial photocurrent, and high sensitivity to O<sub>2</sub>. As for bulk FePor-TPA, its porous structure and high specific surface area make it a possible scaffold to load an amount of AuNPs, the rabbit anti-<i>Staphylococcus aureus</i> Rosenbach tropina antibody (Ab<sub>2</sub>), and GOx for constructing the signal probe (GOx/Ab<sub>2</sub>@Au@FePor-TPA) and realizing catalytic amplification. With these satisfactory features in mind, the 2D FePor-TPA thin film and its bulk powder (FePor-TPA) were utilized to construct a dual and signal-on bioplatform for sensitively and selectively detecting <i>S. aureus</i>, which, as far as we know, has not been reported.
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