Deep spatiotemporal transcriptome analysis provides new insights into early development of koi carp (Cyprinus carpio var. koi)

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 181.5K citations

Life Sciences, Molecular Biology, Molecular Genetics +9

Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2

Michael I. Love, Wolfgang Huber, Simon Anders · Genome biology · 2014 · 100.5K citations · Full text

Life Sciences, Bioinformatics, Biostatistics +15

Cutadapt removes adapter sequences from high-throughput sequencing reads

Marcel Martin · EMBnet journal · 2011 · 35.8K citations · Full text

Life Sciences, Bioinformatics, Molecular Biology +8

HISAT: a fast spliced aligner with low memory requirements

Daehwan Kim, Ben Langmead, Steven L. Salzberg · Nature Methods · 2015 · 26.1K citations · Full text

Engineering, Computer Engineering, Computer Science +3

RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome

Bo Li, Colin N. Dewey · BMC Bioinformatics · 2011 · 23.4K citations · Full text

Life Sciences, Bioinformatics, Molecular Biology +15