New Anti-Hypoxic Metabolites from Co-Culture of Marine-Derived Fungi Aspergillus carneus KMM 4638 and Amphichorda sp. KMM 4639

Abstract

The KMM 4639 strain was identified as <i>Amphichorda</i> sp. based on two molecular genetic markers: ITS and β-tubulin regions. Chemical investigation of co-culture marine-derived fungi <i>Amphichorda</i> sp. KMM 4639 and <i>Aspergillus carneus</i> KMM 4638 led to the identification of five new quinazolinone alkaloids felicarnezolines A-E (<b>1</b>-<b>5</b>), a new highly oxygenated chromene derivative oxirapentyn M (<b>6</b>) and five previously reported related compounds. Their structures were established using spectroscopic methods and by comparison with related known compounds. The isolated compounds showed low cytotoxicity against human prostate and breast cancer cells but felicarnezoline B (<b>2</b>) protected rat cardiomyocytes H9c2 and human neuroblastoma SH-SY5Y cells against CoCl₂-induced damage.