Molecular Characterization and Bioactivities of a Novel Polysaccharide from Phyllostachys pracecox Bamboo Shoot Residues

Abstract

Dietary carbohydrates are unexploited in the by-products of economically valuable <i>Phyllostachys pracecox</i> bamboo shoots. A residue-derived polysaccharide (PBSR1) was aqueously extracted from the processing waste of this bamboo shoot species. Its primary structure and advanced conformation were elucidated by a combined analysis of spectroscopy, chromatography, 2D nuclear magnetic resonance, laser light scattering and atomic microscopy. The results indicated PBSR1 was a triple-helix galactan consisting of →6)-β-D-Gal<i>p</i> and →3)-β-D-Gal<i>p</i> in linear with an 863 KD molecular weight (M_w). The relationship between the radius of gyration (Rg) and intrinsic viscosity ([η]) on M_w were established as R_g = 1.95 × 10^-2M_w^0.52±0.03 (nm) and [η] = 9.04 × 10^-1M_w^0.56±0.02 (mL/g) for PBSR1 in saline solution at 25 °C, which indicated it adopted a triple-helix chain shape with a height of 1.60 ± 0.12 nm supported by a red shift of λ_max in Congo red analysis. The thermodynamic test (TG) displayed that it had excellent thermal stability for the food industry. Further, those unique structure features furnish PBSR1 on antioxidation with EC₅₀ of 0.65 mg/mL on DPPH· and an ORAC value of 329.46 ± 12.1 μmol TE/g. It also possessed pronounced immunostimulation by up-regulating pro-inflammatory signals including NO, IL-6, TNF-α and IL-1β in murine cells. Our studies provided substantial data for the high-valued application of residues and a better understanding of the structure-function relationship of polysaccharide.