Concepedia

Stepwise Optimization of Real-Time RT-PCR Analysis

Nathan A. Maren, James R. Duduit, Debao Huang, Fanghou Zhao, Thomas G. Ranney, Wusheng Liu

Methods in molecular biology · 2023 · 80 citations · 35 references

DOI

Concepts

References

35

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 181.5K citations

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Mutation of the mouse klotho gene leads to a syndrome resembling ageing.

PubMed · 1997 · 66.6K citations

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A new mathematical model for relative quantification in real-time RT-PCR

Michael W. Pfaffl · Nucleic Acids Research · 2001 · 34.9K citations · Full text

, , +12

Analyzing real-time PCR data by the comparative CT method

Thomas D. Schmittgen, Kenneth J. Livak · Nature Protocols · 2008 · 26.7K citations

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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 20.1K citations · Full text

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