Improvement effect of a next-generation probiotic <i>L. plantarum</i>-pMG36e-GLP-1 on type 2 diabetes mellitus <i>via</i> the gut–pancreas–liver axis

Abstract

Next-generation probiotics (NGPs) are currently being investigated as therapeutic agents that impact the gut microbiota and disease development. Glucagon-like peptide-1 (GLP-1) shows an excellent therapeutic effect on diabetes, but has an extremely short half-life <i>in vivo</i>. Here, we constructed a novel and diabetes-specific NGP, the genetically engineered strain <i>Lactobacillus plantarum</i> (<i>L. plantarum</i>)-pMG36e-GLP-1, and evaluated its ameliorative effect on type 2 diabetes mellitus (T2DM) in artificially induced mice and transgenic mice. <i>In vitro</i>, <i>L. plantarum</i>-pMG36e-GLP-1 showed good genetic stability and probiotic characteristics. In the high-fat diet combined with streptozotocin (HFD/STZ)-induced T2DM mice, <i>L. plantarum</i>-pMG36e-GLP-1 relieved the diabetic symptoms, regulated the intestinal microbiota, and reduced the inflammatory reaction in the pancreatic tissue. Meanwhile, the apoptosis of pancreatic islet cells was inhibited, while islet tissue morphology repairs, islet β-cell proliferation, and insulin secretion were all promoted by <i>L. plantarum</i>-pMG36e-GLP-1. Furthermore, a similar effect of the engineered strain on diabetic symptoms and the pancreas was observed in <i>db</i>/<i>db</i> mice, and the metabolism of lipids in the liver was regulated. Together, the findings of this study confirmed the anti-hyperglycemic effect of the engineered strain <i>L. plantarum</i>-pMG36e-GLP-1, providing a promising approach for T2DM treatment.