Free, Conjugated, and Wall-Bound Polyamines in Various Organs of Fruiting Cuttings of<i>Vitis vinifera</i>L. cv. Cabernet Sauvignon

Abstract

Free polyamines, conjugated polyamines, and wall-bound polyamines were analyzed in several organs of the fruiting cuttings of Cabernet Sauvignon at different stages of development. Polyamine composition differed according to tissue and stage of development with wall-bound putrescine predominating in flowers, rachises, stems, and roots. Putrescine conjugates accumulated only in flowers. These polyamines (except wall-bound putrescine in rachises) quickly and dramatically decreased after anthesis, while conjugated diaminopropane accumulated in berries and rachises. Wall-bound diaminopropane of development predominated in shoot stems at anthesis. Polyamines were always found at low levels in all organs at the stage of fruit ripening.