Supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese

Abstract

The study aimed to investigate the effects of honeysuckle extract (HE) on growth performance, serum biochemical indexes, immune organ indexes, gut morphology, and gut microbes in geese. A total of 180 28-day-old Holdobaki geese were randomly divided into three groups. Each group contained 6 replicates (10 geese, with 5 males and 5 females). The BD group was fed the basal diet, the HE1 group was fed the basal diet supplemented with 1 g/kg of HE, and the HE2 group was fed the basal diet supplemented with 2 g/kg of HE. The experiment lasted for 42 days. The results showed that, compared with the BD group, the average daily gain (ADG) of the HE1 and HE2 groups tended to increase (0.05 < <i>P</i> < 0.10), but the average daily feed intake (ADFI) and final body weight (BW) did not differ significantly, and the feed/gain ratio (F/G) was significantly lower (<i>P</i> < 0.01). The bursa index and the thymus index tended to increase (0.05 < <i>P</i> < 0.10), and serum immunoglobulin A (IgA) and immunoglobulin G (IgG) levels increased significantly (<i>P</i> < 0.05). In the HE1 and HE2 groups, the crypt depth (CD) in the jejunum tended to decrease (0.05 < <i>P</i> < 0.10), and the villus height/crypt depth ratio (V/C) increased significantly in the jejunum and the ileum (<i>P</i> < 0.05). According to 16sRNA microbial community diversity analysis, <i>Firmicutes, Bacteroidetes, Proteobacteria</i>, and <i>Actinobacteria</i> were the dominant phyla. The abundance of <i>Firmicutes</i> was significantly decreased (<i>P</i> < 0.01), while that of <i>Bacteroidetes</i> was significantly increased (<i>P</i> < 0.01), in the HE1 and HE2 groups compared with the BD group. <i>Bacteroides barnesiae, Subdoligranulum variabile, Bacteroides plebeius</i>, and <i>Faecalibacterium prausnitzii</i> were the dominant species, and the abundance of <i>B. plebeius</i> and <i>F. prausnitzii</i> was significantly increased (<i>P</i> < 0.05). According to the LEfSe analysis, BD enriched <i>g_Dorea</i> and <i>g_Dehalobacterium</i>; HE1 enriched g_<i>Faecalibacterium, g_Dialister, g_Prevotella, g_Megamonas, g_Phascolarctobacterium, g_Paraprevotella, g_Anaerostipes, g_Staphylococcus, g_Odoribacter, g_Succinivibrio</i>, and <i>g_Sutterella</i>; and HE2 enriched g_<i>Parabacteroides, g_Olsenella, g_human</i>, and <i>g_Rikenella</i>. According to the Spearman correlation analysis, <i>Bacteroides plebeius</i> was positively correlated with final BW, ADG, IgA, IgG, VH (ileum), and V/C (ileum) and was negatively correlated with F/G and CD (ileum); <i>Ruminococcus gnavus</i> was negatively correlated with final BW, ADG, IgA, and IgG. HE supplementation at 1 g/kg improved growth performance, immune performance, gut morphology, and cecal microbes.