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Simultaneous Determination of Xylazine and Its Major Metabolite, 2,6-Dimethylaniline, in Bovine and Swine Kidney by Liquid Chromatography
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1993
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Bovine KidneySwine KidneyLaboratory Animal StudySeparation ScienceGas ChromatographyBioanalysisAnalytical ChemistryLiquid ChromatographyChromatographyAnimal PhysiologyMajor MetaboliteBiochemistryStandard DeviationChromatographic AnalysisPharmacologyAnimal ScienceMass SpectrometryMetabolismMedicinePharmacokineticsDrug Analysis
Abstract A liquid chromatographic (LC) method is described for the simultaneous determination of xylazine (XY) and its major metabolite, 2,6-dimethylaniline (2,6- DMA), in bovine and swine kidney in the 25– 100 ppb range. XY and 2,6-DMA are extracted from kidney with chloroform, followed by cleanup on an acidic Celite 545 column. A μBondapak phenyl column is used for LC separation with UV determination at 225 nm. The mobile phase is a mixture of acetonitrile, water, sodium acetate, and acetic acid. Mean recoveries from bovine kidney were 78.3% for XY, with a standard deviation (SD) of 7.45 and a coefficient of variation (CV) of 9.51 %, and 87.2% for 2,6-DMA, with an SD of 8.38 and a CV of 9.61 %. Mean recoveries from swine kidney were 80.8% for XY, with an SD of 5.92 and a CV of 7.33%, and 86.7% for 2,6-DMA, with an SD of 6.16 and a CV of 7.10%.