An Improved Method for Determination of Serum Albumin and Globulin

Abstract

Abstract A simple and reliable method has been developed for the analysis of serum proteins. Globulins are precipitated from serum with HCI- ethanol. The albumin remains in the supernatant fluid. The albumin fraction is then precipitated by alcoholic sodium acetate. The proteins are redissolved and determined colorimetrically following their reaction with the biuret reagent. Recovery and electrophoretic studies indicate that the method affords a clean-cut separation of albumin from the globulin fraction. Lipemia does not appear to interfere with the analysis.