CYTOKERATIN 20: A NEW MARKER FOR EARLY DETECTION OF BLADDER CELL CARCINOMA

Abstract

No AccessJournal of UrologyClinical Urology: Original Articles1 Dec 1998CYTOKERATIN 20: A NEW MARKER FOR EARLY DETECTION OF BLADDER CELL CARCINOMA V. BUCHUMENSKY, A. KLEIN, R. ZEMER, O.J. KESSLER, S. ZIMLICHMAN, and I. NISSENKORN V. BUCHUMENSKYV. BUCHUMENSKY , A. KLEINA. KLEIN , R. ZEMERR. ZEMER , O.J. KESSLERO.J. KESSLER , S. ZIMLICHMANS. ZIMLICHMAN , and I. NISSENKORNI. NISSENKORN View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)62215-8AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Cytokeratins are constituents of the intermediate filaments of epithelial cells in which they are expressed in various combinations depending on epithelial type and degree of differentiation. Of the 20 known cytokeratins, cytokeratin 19 is expressed in normal urothelium cells, whereas the recently identified cytokeratin 20 (CK-20) is expressed in urothelial carcinoma but not normal urothelium cells. We examine whether CK-20 expression can be used as a bladder tumor marker for transitional cell carcinoma in cells isolated from urine. Materials and Methods: The reverse transcriptase polymerase chain reaction method was used to determine expression of CK-20 in cells separated from urine of patients with bladder carcinoma. Cells were obtained from urine of 192 patients stratified into 3 groups of 21 healthy young volunteers without a history of transitional cell carcinoma, 27 with a negative bladder biopsy for transitional cell carcinoma and 144 with bladder transitional cell carcinoma. The parameters were tumor stage and grade, tumor size, number of tumors, urinary cytology and CK-20. Results: CK-20 amplification band (370 base pairs) was obtained with messenger ribonucleic acid extracted from transitional cell carcinoma cells of bladder tumor. CK-20 in the urine samples of the control group was negative (no false-positive results, specificity 100%). Among the 27 patients with pTo disease CK-20 was negative in 20 (specificity 74.1%). In the 7 patients with positive CK-20 histology showed chronic inflammation in 2, atypical hyperplasia in 3 and metaplasia in 1. In 1 patient who had a known history of transitional cell carcinoma the urothelium was normal. Among 144 patients with bladder transitional cell carcinoma CK-20 was positive in 131. Sensitivity of the method was much higher than urinary cytology (91 versus 56.3%, p <0.0001). We demonstrated no correlation between CK-20 and tumor grade. Conclusions: Our results indicate that CK-20 is a potential marker for bladder cancer. The noninvasive detection method assesses urothelial cells from the voided urine specimen using reverse transcriptase-polymerase chain reaction. The CK-20 marker was significantly more sensitive than urinary cytology. References 1 : Telomerase activity in bladder cancer, bladder washings and in urine. Int. J Oncology1996; 9: 1169. Google Scholar 2 : The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell1982; 31: 11. Crossref, Medline, Google Scholar 3 : Normal human urothelial cells in vitro: proliferation and induction of stratification. Lab. Invest.1994; 71: 583. Medline, Google Scholar 4 : Cytokeratine 20 in human carcinomas A new histodiagnostic marker detected by monoclonal antibodies.. Amer. J. Pathol.1992; 140: 427. Google Scholar 5 : Neuroblastoma cell detection by reverse transcriptase-polymerase chain reaction (RT-PCR) for tyrosine hydroxylase mRNA. Int. J. Cancer1994; 57: 671. Google Scholar 6 : Detection of melanoma cells in peripheral blood by means of reverse transcriptase and polymerase chain reaction. Lancet1991; 338: 1227. Google Scholar 7 : Detection of H-ras gene point mutation in transitional cell carcinoma of human urinary bladder using polymerase chain reaction. Keio. J. Med.1992; 41: 80. Google Scholar 8 : Expression of cytokeratine 20 in urinary cytology of patients with bladder carcinoma. Cancer1998; 82: 349. Google Scholar 9 : Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction. Brit. J. Cancer1995; 71: 278. Google Scholar 10 : Diagnostic accuracy of urinary cytology, and deoxyribonucleic acid flow cytometry and cytology on bladder washings during followup for bladder tumors. J. Urol.1997; 157: 1660. Link, Google Scholar 11 : “Wildcatting” for breakthroughs in urothelial cancer detection and management-a frustrating business. J. Urol.1995; 154: 1348. Link, Google Scholar 12 : Consensus review of the clinical utility of DNA cytometry in bladder cancer. Report of the DNA Cytometry Consensus Conference. Cytometry1993; 14: 478. Google Scholar 13 : Molecular detection of primary bladder cancer by microsatellite analysis. Science1996; 271: 659. Google Scholar 14 : Prediction of response to treatment in superficial bladder carcinoma through pattern of interleukin-2 gene expression. J. Clin. Oncol.1996; 14: 1778. Google Scholar 15 : Telomerase activity in bladder carcinoma and its implication for noninvasive diagnosis by detection of exfoliated cancer cells in urine. Cancer1997; 79: 362. Google Scholar 16 : Significant telomere reduction in human superficial transitional cell carcinoma. Brit. J. Urol.1996; 78: 704. Google Scholar 17 : Bladder cancer risk assessment with quantitative fluorescence image analysis of tumor markers in exfoliated bladder cells. Cancer1993; 72: 2461. Google Scholar 18 : Image cytometric deoxyribonucleic acid analysis of urine specimens as an adjunct to visual cytology in the detection of urothelial cell carcinoma. J. Urol.1993; 149: 42. Link, Google Scholar From the Department of Urology and Laboratory of Molecular Biology, Meir General Hospital, Kfar Saba and Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byRETZ M, LEHMANN J, AMANN E, WULLICH B, RÖDER C and STÖCKLE M (2018) Mucin 7 and Cytokeratin 20 as New Diagnostic Urinary Markers for Bladder TumorJournal of Urology, VOL. 169, NO. 1, (86-89), Online publication date: 1-Jan-2003.de la rosette J, Smedts F, Schoots C, Hoek H and Laguna P (2018) Changing Patterns of Keratin Expression could be Associated with Functional Maturation of the Developing Human BladderJournal of Urology, VOL. 168, NO. 2, (709-717), Online publication date: 1-Aug-2002.ÖGE Ö, KOZACI D and GEMALMAZ H (2018) THE BTA STAT TEST IS NONSPECIFIC FOR HEMATURIA: AN EXPERIMENTAL HEMATURIA MODELJournal of Urology, VOL. 167, NO. 3, (1318-1320), Online publication date: 1-Mar-2002.INOUE T, NAKANISHI H, INADA K, HIOKI T, TATEMATSU M and SUGIMURA Y (2018) REAL TIME REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION OF URINARY CYTOKERATIN 20 DETECTS TRANSITIONAL CELL CARCINOMA CELLSJournal of Urology, VOL. 166, NO. 6, (2134-2141), Online publication date: 1-Dec-2001.LOKESHWAR V and SOLOWAY M (2018) CURRENT BLADDER TUMOR TESTS: DOES THEIR PROJECTED UTILITY FULFILL CLINICAL NECESSITY?Journal of Urology, VOL. 165, NO. 4, (1067-1077), Online publication date: 1-Apr-2001.GÜDEMANN C, WEITZ J, KIENLE P, LACROIX J, WIESEL M, SODER M, BENNER A, STAEHLER G and DOEBERITZ M (2018) DETECTION OF HEMATOGENOUS MICROMETASTASIS IN PATIENTS WITH TRANSITIONAL CELL CARCINOMAJournal of Urology, VOL. 164, NO. 2, (532-536), Online publication date: 1-Aug-2000. Volume 160Issue 6 Part 1December 1998Page: 1971-1974 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information V. BUCHUMENSKY More articles by this author A. KLEIN More articles by this author R. ZEMER More articles by this author O.J. KESSLER More articles by this author S. ZIMLICHMAN More articles by this author I. NISSENKORN More articles by this author Expand All Advertisement PDF downloadLoading ...