Nanoscale · 2022 · 11 citations · 74 references
EngineeringBioengineeringMolecular BiologyMolecular BioengineeringMolecular BiotechnologySynthetic BiologyBiological MembranesNucleic Acid DeliveryVesicle FusionElectroporationLipid VesiclesSynthetic AnaloguesSynthetic Nucleic Acid Analogues
DNA-assisted selective electrofusion (DASE) combines the efficiency of standard electrofusion with the selectivity of DNA-mediated interactions. Here we apply DASE to induce the fusion between giant lipid vesicles and E. coli derived spheroplasts.
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Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson, John I. Glass, Carole Lartigue et al. · Science · 2010 · 2.7K citations · Full text
Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
Yu Jiang, Biao Chen, Chunlan Duan et al. · Applied and Environmental Microbiology · 2015 · 1.4K citations · Full text