Clinical Chemistry · 1968 · 296 citations · 0 references
PharmacyBiochemistryNephrologyElemental AnalysisFood AnalysisMetabolic StabilityBioanalytical Method ValidationColor AnalysisRenal Function TestingRenal Function TestsBiological Sample StabilityColor DevelopmentCreatinine Analysis In Body FluidsCreatinine Assay InterferenceCreatinine-based BiomarkersSample Stability AssessmentSerum Creatinine Assessment
Abstract Total chromogen, true, and AutoAnalyzer methods of measuring serum and urine creatinine by the Jaffe reaction were investigated. Some factors influencing this reaction were examined. These included wavelength, blank, linearity, and conditions of color development. Modifications of the three methods were made and their precision, recovery, and sample stability determined. The interference of ketones and glucose were measured. Finally, the values obtained by the three methods on the same samples of serum and urine were compared statistically.